An Iron-Containing Superoxide Dismutase from the chemolithotrophic Thiobacillus denitrificans ˇ+ RT ˇ; Strain
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چکیده
Superoxide dismutase has been purified to homogeneity from aerobically grown Thiobacillus denitrijicarzs strain “RT”. It has a molecular weight of 43,000, is composed of two identical subunits which are not covalently bound, and contains 1.35 atom of iron per molecule. Absorption spectra and amino acid analysis are similar to those of other Fe-superoxide dismutases from bacteria. Aerobically and anaerobically grown cells contain the same Fe-enzyme with similar levels of activity. Manometric sulfite oxidation measurements suggest for the enzyme a protective function of sulfite against the autooxidation initiated by superoxide free radicals.
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